cxcl9  (Proteintech)

Journal: bioRxiv

Article Title: Translational toolkit for reproducible, cross-study profiling of human ageing hallmarks in human blood and tissue

doi: 10.64898/2026.04.20.719545

Figure Lengend Snippet: A. Schematic overview of a novel 32-color spectral flow cytometry panel for profiling circulating immune populations and intracellular senescence markers p16 and p21. B. PCA analysis of young and aged peripheral blood mononuclear cells (PBMCs), integrating all identified immune cell subsets. C. IMMAge score calculated based on PBMC subsets for young and aged participants in young (n=8) and aged (n=12) participants. D. CD4+ CD57+CD28- and E. CD8+ CD57+CD28-senescent cells expressed as a percentage of total CD4+ or CD8 T cells in young (n=8) and aged (n=12) participants. Intracellular p21 expression in F. Naïve and G. EMRA CD8+ T cells from (n=9) young and aged (n=12) participants. H. Intracellular p16 expression assessed as median fluorescent intensity in Memory B cells (CD19+ CD38− CD24+) from (n=9) young and aged (n=12) participants. Expression of CD95 in I. CD4+ and J. CD8+ T cells between in young (n=8) and aged (n=12) participants. K. Schematic overview of cryosection and whole-tissue approaches for detecting cellular senescence using β-galactosidase staining (SA-beta gal) in human adipose tissue. Representative images of positive β-galactosidase staining (blue) of senescent cells in L. 20 µM human adipose tissue cryosections and M. whole human adipose tissue following 24h incubation. Expression of SASP markers N. GDF15, O. CXCL-9, P. TNF alpha, and Q. IL-6 in plasma samples from young (n=5) and aged (n=12) participants, determined by ELISA. Data are mean ± S.E.M. Statistical significance was assessed (C-F, H-J) unpaired t-tests or (G, N-Q) Mann Whitney U.

Article Snippet: Serum and EDTA plasma concentration of CXCL-9, GDF-15, IL-6 and TNFα, were measured using commercially available ELISA kits (CXCL9; DY392-05, GDF15; DY957, IL-6; DY206, TNF; DY210, R&D Systems, MN, USA) following the manufacturer’s protocol.

Techniques: Flow Cytometry, Expressing, Staining, Incubation, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Cell Death & Disease

Article Title: Mitochondrial DNA release via VDAC1 in keratinocytes: a key driver of innate immunity and vitiligo pathogenesis

doi: 10.1038/s41419-026-08585-5

Figure Lengend Snippet: A–C Time-course analysis (1, 3, 6 h) of 1 μg/mL mtDNA transfection-induced effects in HaCaT cells. A Western blot analysis of cGAS, STING, p-NF-κB, IFN-γ, CXCL9, and CXCL10. B Transcript levels of IFN-α, IFN-β, IFN-γ, CXCL9, CXCL10, IL-6, and IL-1β by RT-qPCR. C Western blot analysis of NLRP3, cleaved Caspase-1 and GSDMD-N protein expression. D–I HaCaT cells transfected with 1 μg/mL mtDNA ±10 μM RU.521 (cGAS inhibitor, 1 h pretreatment). D Representative image of PI/Calcein AM staining. Scale bars, 100 μm. E Quantification of PI-positive cells in three randomly chosen fields (one field per well). F LDH release in culture supernatants. G Secretion level of CXCL9 and CXCL10 in culture supernatants by ELISA. H Transcript levels of IFN-α, IFN-β, IFN-γ, CXCL9, CXCL10, IL-6, and IL-1β by RT-qPCR. I CXCL10 expression by immunofluorescence. Scale bars, 50 μm. Data are presented as mean ± SD ( n = 3). Asterisks * indicate a significant difference exists between indicated groups, * P < 0.05, ** P < 0.01, *** P < 0.001. VEH vehicle, GSDMD-N gasdermin D N-terminal fragment, PI propidium iodide, LDH lactate dehydrogenase.

Article Snippet: The levels of CXCL9 and CXCL10 in human samples were quantified using the corresponding ELISA kits (CXCL9: Elabscience, Hubei, China, E-EL-H6062; CXCL10: Elabscience, E-EL-H0050), following the manufacturer’s protocols.

Techniques: Transfection, Western Blot, Quantitative RT-PCR, Expressing, Staining, Enzyme-linked Immunosorbent Assay, Immunofluorescence